Coding

Part:BBa_K925005:Design

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)


PdTagGST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 292
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the sequence for this part we had to consider the vector we would use as we required one that already contained GST. This allowed us to design primers that would anneal to each other to make the short peptide sequence and use this as our insert.


Source

This part was biologically synthesised.

References